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:: Volume 6, Issue 1 (Spring 2019) ::
2019, 6(1): 59-67 Back to browse issues page
Bioinformatics Study of the Effect of Brain-derived Neurogenic Factor (BDNF) on Gene Expression in SH-SY5Y Cell Line
Korosh Bamdad , Fereshteh Dadfar , Shahnaz Eshraghi
Ph.D., in Animal physiology, Assistant Professor, Biology Dept., Faculty of Sciences, Payame Noor University, Tehran, Iran
Abstract:   (1887 Views)
Introduction: Considering the importance of the evaluation and identification of BDNF protective pathways, this study was conducted to analyze the expression rate of genes registered in the NCBI database to identify the genes expressed in SH-SY5Y cell line due to BDNF protection and oxidative stress and also to identify the protective pathways of BDNF.
Method: In this study, bioinformatics and NCBI databases and libraries including 48000 datasets were explored and data were collected using Illumina and Bid studio software. In the first phase, sampling was performed manually based on the P-value, and in the second phase, based on the relationship with compatibility of neurons, the Pearson correlation coefficient, Spearman's correlation coefficient, and linear relationship  were calculated by measuring fit or regression using SPSS version 20.
Results: The Pearson correlation between CMP and CTR data was positive; and the linear regression between them was also positive. The frequency percentage of neuron adapter proteins obtained from CTR data was higher than that from CMP data; and a great number of protective proteins were related to the protection of cell shape and cellular skeleton, and neuron survival.
Conclusion: Due to the contact of neurons with BDNF, some genes are specifically expressed, therefore, BDNF can increase the life and compatibility of neurons.
Keywords: Neuron, Brain-derived neurogenic factor, SH-SY5Y, NCBI
eprint link: http://eprints.kmu.ac.ir/id/eprint/31286
Full-Text [PDF 1292 kb]   (360 Downloads)    
Type of Study: Original Article | Subject: Bioinformatics
Received: 2018/09/8 | Accepted: 2018/10/6
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Bamdad K, Dadfar F, Eshraghi S. Bioinformatics Study of the Effect of Brain-derived Neurogenic Factor (BDNF) on Gene Expression in SH-SY5Y Cell Line. Journal of Health and Biomedical Informatics. 2019; 6 (1) :59-67
URL: http://jhbmi.ir/article-1-342-en.html

Volume 6, Issue 1 (Spring 2019) Back to browse issues page
مجله انفورماتیک سلامت و زیست پزشکی Journal of Health and Biomedical Informatics
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